JMS-S3000 SpiralTOF™-plus 2.0 Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometer Model：JMS S3000 MALDI-TOF

To improve the mass resolving power and mass accuracy of a time-of-flight mass spectrometer, the flight distance must be extended while keeping a group of ions having the same m/z (an ion packet) from diverging in space.
The innovative SpiralTOF ion optics was developed by JEOL based on the "Perfect focusing" and "Multi-turn" principles. The ion packets are focused back in space at every fixed distance (i.e., each figure-eight trajectory) during the flight. Thus, even after the extended flight distance, the ion packets do not diverge at the detection plane, achieving high mass resolving power, high mass accuracy, and high ion transmission.
 

The topographic effect of the matrix crystal leads to a difference in flight start position for the ions, resulting in a difference in flight time. In the conventional ion optical system, this time difference degrades the mass resolving power and also the mass accuracy obtained with external mass calibration. With its extended flight distance, the JMS-S3000 reduces this effect to the minimum and achieves highly reproducible mass resolving power and high mass accuracy with an external mass calibration. High mass-resolution and mass accuracy can be maintained for imaging analysis of a biological specimen in which a large number of mass spectra are acquired across a large area and the specimen surface is likely to be uneven.

The SpiralTOF™-plus 2.0 has realized a wide dynamic range by greatly improving the detection system. This makes it possible to simultaneously detect peaks with ion intensity differences of about 4 orders of magnitude. Also, the analysis of trace components has become easy in mass spectrometry imaging measurements, in addition to the conventional bulk sample measurements. Below is the measurement example of a mixture of polyethylene oxide and polypropylene oxide in the ratio of 1,000:1. In the case of polymer analysis, when combined with the Kendrick Mass Defect (KMD) analysis, it is possible to analyze trace components that are otherwise difficult to detect.

The mass spectrum of a mixture of polyethylene oxide and polypropylene oxide in the ratio of 1,000:1
The SpiralTOF™-plus 2.0 can realize a wide dynamic range and calculate the molecular weight distribution of trace components.

Features and usages of TOF-TOF option and linear TOF option

 

• By adopting the SpiralTOF ion optics as the first MS, the high precursor ion selectivity can be realized. The monoisotopic peak of precursor ions can be properly selected.
• High-energy collision-induced dissociation (HE-CID) allows for the acquisition of product ion mass spectrum rich with structural information.
• Off set parabolic reflectron, JEOL's patented technology, enables acquisition of all product ion information from m/z 5 to the precursor ion, and facilitates to obtain structural information of high reliability.

Usage

Linear TOF option

• In the Linear TOF option, the ions travel from the ion source to the detector unaffected.
• When ions undergo post source decay (PSD) in flight, the produced fragment ions and neutrals continue to fly at the same velocity as before fragmentation. Hence, in a Linear mode mass spectrum, they are detected as the same signal as that of the ions that have not fragmented.
  Therefore high molecular weight compounds that tend to undergo PSD can be measured with high sensitivity in the Linear mode.
• The combination of Spiral and Linear modes further expands the range of analytes that can be measured.

Usage

• Useful for screening of molecular weight distribution of polymers.
• It is possible to calculate the molecular weight distribution & polydispersity of polymer samples with various masses ranging from several thousands to several tens of thousands.
• It is possible to measure high-mass samples of molecular weight over 10,000 Da such as intact proteins, with high sensitivity.
• It enables high-sensitivity measurement of samples that can easily undergo PSD, such as proteins and polysaccharides.

 

msRepeatFinder: the Definitive Polymer Analysis Software (Option)

SpiralTOF™-plus 2.0 is Ideal for Polymer Analysis
Industrial polymeric materials based on mixtures of polymers with different end groups or copolymers contain a wide variety of compounds. It is necessary to detect all the components in order to grasp the whole picture, which requires ultra-high mass resolution in a wide mass range. In addition, it is important to detect not only the base material but also trace components because multiple types of polymers and trace additives are blended for higher functionality.
With its wide dynamic range and ultra-high mass resolution over a wide mass range, SpiralTOF™-plus 2.0 is the solution that meets these requirements.
Elimination of post source decay (PSD) derived ions, which is a major feature of SpiralTOF ion optics, also contributes significantly to clear mass spectrum analysis.
We provide the most effective and unique solution for polymer analysis, which has become more and more complicated in recent years due to its high functionality and recycling.


The SpiralTOF™-plus 2.0 achieves high mass resolving power over a wide mass range which is required for the analysis of polymers.

The Kendrick Mass Defect (KMD) plot and the Kendrick Mass Remainder (KMR) plot are used to estimate the polymer species and end groups contained in polymer materials from a complex mass spectrum and clarify their identity. In addition, the differential analysis function between two samples is effective in verifying sample degradation, lot-to-lot differences, and differences in the synthesis process.
 msRepeatFinder Polymer Analysis Software

Evolving mass imaging analysis

Mass spectrometry imaging of polymers

For the detailed analysis of a protein, not only the intact protein but also enzymatic digest of the protein can be analyzed. Peptide mass fingerprinting allows identification of a protein.
Mass spectrum of the tryptic digest of bovine serum albumin (BSA) and the results of the peptide mass fingerprinting.
Mass spectrum of BSA tryptic digest standard (equivalent to 500 amol)

 

Amount (fmol)	Number of peptides matched/searched	Sequence coverage (%)	MASCOT score
50	52/81	75	570
10	41/79	64	390
5	36/77	54	351
1	28/57	43	255
0.5	31/52	46	306
0.1	12/34	18	92

 

Analysis of small molecules